Long-term studies on West Antarctic Peninsula phytoplankton blooms suggest range shifts between temperate and polar species.

The Western Antarctic Peninsula (WAP) experiences one of the highest rates of sea surface warming globally, leading to potential changes in biological communities. Long-term phytoplankton monitoring in Potter Cove (PC, King George Island, South Shetlands) from the 1990s to 2009 revealed consistently low biomass values, and sporadic blooms dominated by cold-water microplankton diatoms. However, a significant change occurred between 2010 and 2020, marked by a notable increase in intense phytoplankton blooms in the region. During this period, the presence of a nanoplankton diatom, Shionodiscus gaarderae, was documented for the first time. In some instances, this species even dominated the blooms. S. gaarderae is recognized for producing blooms in temperate waters in both hemispheres. However, its blooming in the northern Southern Ocean may suggest either a recent introduction or a range shift associated with rising temperatures in the WAP, a phenomenon previously observed in experimental studies. The presence of S. gaarderae could be viewed as a warning sign of significant changes already underway in the northern WAP plankton communities. This includes the potential replacement of microplankton diatoms by smaller nanoplankton species. This study, based on observations along the past decade, and compared to the previous 20 years, could have far-reaching implications for the structure of the Antarctic food web.

Necrosis pancreática infectada: tratamiento solo con antibióticos. ¿Sería el primer paso del step-up approach? / Infected pancreatic necrosis: Treatment with antibiotics alone. Would this be the first step of the step-up approach?

Resumen Introducción: En 1994, Claudio Bassi relató un caso de tratamiento médico de la necrosis pancreática infec tada (NPI); luego desde 1996 se publicaron numerosos artículos de serie de casos con tratamiento solo con antibióticos con buenos resultados. Objetivos: Presentar nuestra experiencia en el manejo de la necrosis pancreática infectada con antibióticos (sin drenaje). Métodos: Revisamos retrospectivamente los pacientes con diagnóstico de NPI desde enero de 2018 a octubre del 2020, enfocándonos en aquellos casos que se trata ron de forma conservadora (soporte hidroelectrolítico, nutricional y antibióticos). El diagnóstico se realizó ob servando gas en el retroperitoneo por TC asociado o no a deterioro clínico del paciente con necrosis pancreática sin otro foco. No se realizó punción aspiración con aguja fina (PAAF). Resultados: Identificamos 25 pacientes con diag nóstico de NPI. Once fueron tratados de forma conser vadora. Según la clasificación de Atlanta, modificada en 2012, 3 casos fueron clasificados de forma grave y el resto moderadamente grave. Todos recibieron antibióticos al menos durante 3 semanas. Ninguno requirió nutrición parenteral. El promedio de estan cia hospitalaria fue de 38 días. Tres pacientes fueron readmitidos. A 8 se les realizó colecistectomía luego de haber resuelto el cuadro; los restantes ya estaban colecistectomizados. No hubo muertes en esta serie. Conclusiones: La NPI puede ser tratada de forma con servadora sin drenaje con buenos resultados en casos seleccionados.

Abstract Introduction: In 1994, Claudio Bassi reported a case of medical treatment for infected pancreatic necrosis (IPN); then since 1996 numerous articles of case series were published with treatment only with antibiotics with good outcomes. Objectives: To present our experience in the mana gement of patients with IPN with antibiotics (without drainage). Methods: We retrospectively reviewed cases with a diagnosis of IPN from January 2018 to October 2020, focusing on those cases that were treated conservatively (hydro-electrolyte, nutritional support and antibiotics). The diagnosis was made by observing gas in the re troperitoneum by CT or by clinical deterioration of the patient with pancreatic necrosis without another focus. Fine needle aspiration was not performed. Results: We identified 25 patients with a diagnosis of IPN; eleven were treated conservatively. According to Atlanta, modified in 2012, 3 were classified severely and the rest moderately severe. All received antibiotics for at least 3 weeks. None required parenteral nutrition. The mean hospital stay was 38 days. Three patients were readmitted. 8 underwent cholecystectomy after having resolved the condition; the rest were already cholecys tectomized. There were no deaths in this series. Conclusions: IPN can be treated conservatively without drainage with good results in selected cases.

[Infected pancreatic necrosis: Treatment with antibiotics alone. Would this be the first step of the step-up approach?] / Necrosis pancreática infectada: tratamiento solo con antibióticos. ¿Sería el primer paso del step-up approach?

INTRODUCTION: In 1994, Claudio Bassi reported a case of medical treatment for infected pancreatic necrosis (IPN); then since 1996 numerous articles of case series were published with treatment only with antibiotics with good outcomes. OBJECTIVES: To present our experience in the management of patients with IPN with antibiotics (without drainage). METHODS: We retrospectively reviewed cases with a diagnosis of IPN from January 2018 to October 2020, focusing on those cases that were treated conservatively (hydro-electrolyte, nutritional support and antibiotics). The diagnosis was made by observing gas in the retroperitoneum by CT or by clinical deterioration of the patient with pancreatic necrosis without another focus. Fine needle aspiration was not performed. RESULTS: We identified 25 patients with a diagnosis of IPN; eleven were treated conservatively. According to Atlanta, modified in 2012, 3 were classified severely and the rest moderately severe. All received antibiotics for at least 3 weeks. None required parenteral nutrition. The mean hospital stay was 38 days. Three patients were readmitted. 8 underwent cholecystectomy after having resolved the condition; the rest were already cholecystectomized. There were no deaths in this series. CONCLUSIONS: IPN can be treated conservatively without drainage with good results in selected cases.

Introducción: En 1994, Claudio Bassi relató un caso de tratamiento médico de la necrosis pancreática infectada (NPI); luego desde 1996 se publicaron numerosos artículos de serie de casos con tratamiento solo con antibióticos con buenos resultados. OBJETIVOS: Presentar nuestra experiencia en el manejo de la necrosis pancreática infectada con antibióticos (sin drenaje). Métodos: Revisamos retrospectivamente los pacientes con diagnóstico de NPI desde enero de 2018 a octubre del 2020, enfocándonos en aquellos casos que se trataron de forma conservadora (soporte hidroelectrolítico, nutricional y antibióticos). El diagnóstico se realizó observando gas en el retroperitoneo por TC asociado o no a deterioro clínico del paciente con necrosis pancreática sin otro foco. No se realizó punción aspiración con aguja fina (PAAF). RESULTADOS: Identificamos 25 pacientes con diagnóstico de NPI. Once fueron tratados de forma conservadora. Según la clasificación de Atlanta, modificada en 2012, 3 casos fueron clasificados de forma grave y el resto moderadamente grave. Todos recibieron antibióticos al menos durante 3 semanas. Ninguno requirió nutrición parenteral. El promedio de estancia hospitalaria fue de 38 días. Tres pacientes fueron readmitidos. A 8 se les realizó colecistectomía luego de haber resuelto el cuadro; los restantes ya estaban colecistectomizados. No hubo muertes en esta serie. CONCLUSIONES: La NPI puede ser tratada de forma conservadora sin drenaje con buenos resultados en casos seleccionados.

The Reovirus σ1 Attachment Protein Influences the Stability of Its Entry Intermediate.

Structural metastability of viral capsids is pivotal for viruses to survive in harsh environments and to undergo timely conformational changes required for cell entry. Mammalian orthoreovirus (reovirus) is a model to study capsid metastability. Following initial disassembly of the reovirus particle mediated by proteases, a metastable intermediate called the infectious subvirion particle (ISVP) is generated. Using a σ1 monoreassortant virus, we recently showed that σ1 properties affect its encapsidation on particles and the metastability of ISVPs. How metastability is impacted by σ1 and whether the lower encapsidation level of σ1 is connected to this property is unknown. To define a correlation between encapsidation of σ1 and ISVP stability, we generated mutant viruses with single amino acid polymorphisms in σ1 or those that contain chimeric σ1 molecules composed of σ1 portions from type 1 and type 3 reovirus strains. We found that under most conditions where σ1 encapsidation on the particle was lower, ISVPs displayed lower stability. Characterization of mutant viruses selected for enhanced stability via a forward genetic approach also revealed that in some cases, σ1 properties influence stability without influencing σ1 encapsidation. These data indicate that σ1 can also influence ISVP stability independent of its level of incorporation. Together, our work reveals an underappreciated effect of the σ1 attachment protein on the properties of the reovirus capsid. IMPORTANCE Reovirus particles are comprised of eight proteins. Among them, the reovirus σ1 protein functions engages cellular receptors. σ1 also influences the stability of an entry intermediate called ISVP. Here, we sought to define the basis of the link between σ1 properties and stability of ISVPs. Using variety of mutant strains, we determined that when virus preparations contain particles with a high amount of encapsidated σ1, ISVP stability is higher. Additionally, we identified portions of σ1 that impact its encapsidation and consequently the stability of ISVPs. We also determined that in some cases, σ1 properties alter stability of ISVPs without affecting encapsidation. This work highlights that proteins of these complex particles are arranged in an intricate, interconnected manner such that changing the properties of these proteins has a profound impact on the remainder of the particle.

Sex-Specific Association between Fasting Plasma Glucose and Serum Selenium Levels in Adults from Southern Mexico.

Selenium (Se) is an essential trace element that by its antioxidant properties has been studied to elucidate its participation in the development of obesity and type 2 diabetes. We evaluated the association between cardiometabolic traits and serum Se levels in a sample of adults from southern Mexico. In 96 nondiabetic individuals, anthropometric data and clinical biochemistry measurements were analyzed. Serum total Se levels were measured with inductively coupled plasma mass spectrometry (ICP-MS). Serum Se level in the whole sample was 10.309 ± 3.031 µg mL-1 and no difference between the women and men was observed (p = 0.09). Additionally, fasting plasma glucose (FPG) was significantly associated with serum Se level (ß = -0.07 ± 0.03, p = 0.02, analysis adjusted for age, sex and BMI). Furthermore, sex shows significant interaction with FPG on the serum Se levels (p = 0.01). A follow-up analysis revealed the particular association between FPG and Se levels in women (ß = -0.10 ± 0.04, p = 0.01). In conclusion, our data evidenced a women-specific association between FPG and serum Se levels in a sample of adults from southern Mexico.

Serum zinc levels are associated with obesity and low-density lipoprotein cholesterol in Mexican adults.

PURPOSE: Little is known about the association between serum zinc (Zn) levels and obesity in the Mexican population. Therefore, we tested the association between serum Zn levels, obesity status, and serum lipid levels in a sample of Mexican adults. METHODS: Anthropometric data and serum levels of total cholesterol, high- and low-density lipoprotein cholesterol (HDL-C and LDL-C, respectively), and triglycerides were analyzed in 96 Mexican adults. Serum Zn was measured with inductively coupled plasma mass spectrometry. An individual data meta-analysis of the association between serum Zn, overweight, and obesity status was performed in 172 adults from two different provinces in Mexico. RESULTS: Serum Zn was negatively associated with body mass index (BMI, ß = -0.034 ± 0.013, p = 2.0 ×10-6) and obesity (odds ratio [OR]= 0.990, 95% confidence interval [CI]= 0.980-0.999, p = 3.4 ×10-5). The association between Zn level and obesity in Mexican adults was confirmed with an individual data meta-analysis (OR= 0.977, 95% CI= 0.966-0.988, p = 3.4 ×10-5). In addition, a significant interaction effect between serum Zn level and sex was observed on LDL-C level (ß = 7.010 ± 3.295, p = 0.037). Serum Zn was negatively associated with LDL-C levels in women (ß = -0.188 ± 0.074, p = 0.016). CONCLUSION: Our results confirm the negative association of serum Zn level with obesity. For the first time, we show a sex-specific association between serum Zn and LDL-C levels in a Mexican population. However, further studies are needed in larger and more varied Mexican cohorts to replicate and confirm our findings.

Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.

The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.

Uso de nuevos anticoagulantes en la Unidad Coronaria del Hospital Privado del Sur de Bahía Blanca / Use of news anticoagulants in the Coronary Unit of Hospital Privado del Sur in Bahía Blanca

Introducción: La utilización de los nuevos anticoagulantes orales (NACOs), en reemplazo de los clásicos antagonistas de la vitamina K (AVK), es una nueva opción terapéutica para el manejo de enfermedades tromboembólicas. Objetivo: Iniciar un estudio retrospectivo acerca de la utilización de los NACOS con pacientes del Servicio de Cardiología del HPS, para disponer de datos propios respecto al uso de estos nuevos anticoagulantes. Materiales y Métodos: Estudio retrospectivo, observacional y longitudinal, con la base de datos del Servicio de Cardiología del HPS (Junio de 2016-Mayo de 2017). Población: pacientes con Fibrilación Auricular (FA) y Tromboembolismo pulmonar (TEP) tratados con NACOs. La recolección de datos fue mediante encuesta telefónica voluntaria a pacientes o familiares con un formulario preestablecido. Resultados: Se registró un evento hemorrágico mayor en un paciente con bajo riesgo hemorrágico. No se registró ningún evento cardioembólico. Discusión: Los nuevos anticoagulantes orales resultaron ser seguros en cuanto a sus complicaciones y mejor aceptados por los pacientes que los AVK. La principal limitante de la continuidad de su uso fue la diferencia de costos, respecto a los clásicos antagonistas de la Vitamina K.

Micro- and mesozooplankton successions in an Antarctic coastal environment during a warm year.

The rapid increase in atmospheric temperature detected in the last decades in the Western Antarctic Peninsula was accompanied by a strong glacier retreat and an increase in production of melting water, as well as changes in the sea-ice dynamic. The objective of this study was to analyze the succession of micro- and mesozooplankton during a warm annual cycle (December 2010-December 2011) in an Antarctic coastal environment (Potter Cove). The biomass of zooplankton body size classes was used to predict predator-prey size relationships (i.e., to test bottom-up/top-down control effects) using a Multiple Linear Regression Analysis. The micro- and mesozooplanktonic successions were graphically analyzed to detect the influence of environmental periods (defined by the degree of glacial melting, sea-ice freezing and sea-ice melting) on coupling/uncoupling planktonic biomass curves associated to possible predator-prey size relationship scenarios. At the beginning of the glacial melting, medium and large mesozooplankton (calanoid copepods, Euphausia superba, and Salpa thompsoni) exert a top-down control on Chl-a and microzooplankton. Stratification of the water column benefitted the availability of adequate food-size (Chl-a <20) for large microzooplankton (tintinnids) development observed during fall. High abundance of omnivores mesozooplankton (Oithona similis and furcilia of E. superba) during sea-ice freezing periods would be due to the presence of available heterotrophic food under or within the sea ice. Finally, the increase in microzooplankton abundance in the middle of spring, when sea-ice melting starts, corresponded to small and medium dinoflagellates and ciliates species, which were possibly part of the biota of sea ice. If glacier retreat continues and the duration and thickness of the sea ice layer fluctuates as predicted by climate models, our results predict a future scenario regarding the zooplankton succession in Antarctic coastal environments.

Cardiomiopatía de Tako-tsubo y síndrome de QT largo congénito / Tako-tsubo cardiomyopathy and congenital long QT syndrome

La miocardiopatía de Tako-tsubo (MT) y síndrome de QT largo congénito (SQTLc) son dos entidades que presentan un retardo de la repolarización cardíaca y su asociación ha sido publicada en reportes de casos. Presentamos el caso de una paciente de 55 años, con antecedentes de SQTLc, que ingresó a nuestro hospital por dolor precordial, luego de un evento estresante, diagnosticándose MT. Evolucionó con prolongación del intervalo QT en el electrocardiograma, persistiendo luego de la externación, sin presentar episodios de arritmias ventriculares ni síncope

Takobsubo cardiomyopathy (TC) and congenital long QT syndrome (LQTS) are two entities that present a delay of cardiac repolarization and their association has been published in case reports. We present the case of a 55-year-old female patient with medical history of congenital LQTS, who was admitted to our hospital due to precordial pain after a stressful event and TC was diagnosed. She evolves with QT interval prolongation on the ECG that remains after the externalization, without presenting any episodes of ventricular arrhythmia or syncope

A cardiomiopatia de Tako-tsubo (CT) e a síndrome do QT longo congênito (SQTLc) são duas entidades que apresentam um atraso da repolarização cardíaca e sua associação foi publicada em relatos de casos. Apresentamos o caso de uma paciente de 55 anos de idade com história médica de SQTLc, admitida em nosso hospital devido a dor precordial após um evento estressante, com diagnóstico de CT. Ela evolui com prolongamento do intervalo QT no ECG que permanece após a externalização, sem apresentar nenhum episódios de arritmias ventriculares ou síncope

Muerte súbita cardíaca asociada a puente miocárdico / Sudden cardiac death associated with myocardial bridge

La muerte súbita cardíaca en deportistas genera un gran impacto social y familiar, afortunadamente su incidencia es baja. En atletas menores de 35 años las causas más frecuentes son las anomalías genéticas y las miocardiopatías. Presentamos el caso de un paciente masculino de 17 años que presentó muerte súbita reanimada mientras realizaba actividad física. El electrocardiograma post animación evidenció una injuria subepicárdica en cara anterior, encontrándose en la cinecoronariografía como único hallazgo, la existencia de un puente muscular en tercio medio de la arteria descendente anterior.

Cardiac arrest in athletes has a high impact in family and society, fortunately its incidence is low. In athletes younger than 35 years old the most frequent causes are genetic anomalies and cardiomyopathies. We report the case of a 17 years old male patient who presented cardiac arrest and reanimation while doing physical activity. The post-reanimation electrocardiogram showed a subepicardial injury in the anterior face, detecting in cinecoronariography as only finding the presence of myocardial bridging in the middle third of anterior descending artery.

A parada cardíaca em atletas tem um alto impacto na família e na sociedade, felizmente sua incidência é baixa. Em atletas menores de 35 anos as causas mais freqüentes são anomalias genéticas e cardiomiopatías. Relatamos o caso de um paciente do sexo masculino de 17 anos de idade que apresentou parada cardíaca e reanimação durante atividade física. O eletrocardiograma pós-reanimação mostrou lesão subepicárdica na face anterior, detectar na cinecoronariografia como apenas encontrar a presença de ponte miocárdica no terço médio da artéria descendente anterior.

Taquicardia ventricular como manifestación de sarcoidosis cardíaca. Reporte de un caso / Ventricular tachycardia as a manifestation of cardiac sarcoidosis. Case report

La sarcoidosis es una enfermedad inflamatoria crónica que posee una amplia variabilidad de presentaciones clínicas. El compromiso cardíaco de esta patología oscila en el orden del 5%, pudiendo variar desde anormalidades en la conducción hasta la falla cardíaca. Existe poco conocimiento sobre los beneficios de su tratamiento y no hay datos que evalúen la reducción de la mortalidad a largo plazo, por lo que genera este tipo de pacientes un gran desafío para el profesional. A continuación presentamos el caso de un masculino de 37 años con taquicardia ventricular como debut cardiológico de una sarcoidosis.

Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins.

A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.

Registro de pacientes con insuficiencia cardíaca aguda en la unidad coronaria del Hospital Privado del Sur de Bahía Blanca / Recording of patients with acute heart failure in the coronary care unit at Hospital Privado del Sur in Bahía Blanca

Introducción: La insuficiencia cardíaca (IC) es una entidad clínica con alta prevalencia que implica una elevada morbi-mortalidad. Conocer nuestros registros es una herramienta fundamental para intervenir en el abordaje de esta patología. Objetivo: Analizar el registro de pacientes ingresados a la unidad coronaria (UCO) del Hospital Privado del Sur de Bahía Blanca (HPS), que fueron diagnosticados con IC. Material y Métodos: Estudio descriptivo transversal de los pacientes ingresados a UCO del HPS durante el período julio 2016 -julio 2017 con diagnóstico de alta de IC. De la base de datos de UCO del HPS se obtuvieron las siguientes variables: edad, sexo, factores de riesgo cardiovascular (FRC), causas desencadenantes, comorbilidades, fracción de eyección (FEY), necesidad de inotrópicos y asistencia respiratoria mecánica, días de internación y número de reinternaciones. Análisis estadísticos: En el análisis de variables cualitativas se utilizaron frecuencias absolutas y relativas. Para variables cuantitativas se empleó la mediana. La recopilación y análisis estadístico se realizó con el programa informático SPSS.Resultados: Se registraron 85 pacientes, 58 hombres (68,2%); 70 (82,4%) fueron mayores de 65 años; Patologías: 66 (77,6 %) hipertensos, 27 (31,8 %) diabéticos, 37 (43,5 %) dislipémicos, 30 (35,3 %) tenían antecedentes de IAM, 17 (20,0 %) de fibrilación auricular. Las causas desencadenantes más frecuentes fueron transgresión dietaria, arritmia e infección. Tuvieron Fraccione de Eyección (FEY) menor de 40 %: 45 pacientes (52,9 %). La mediana de días de internación fue 5 (2-53); 7 (8,2%) pacientes se reinternaron, cuya mediana fue 1 (Score 1-5). Conclusiones: Entre las causas desencadenantes, los Factores de Riesgos Cardiovascular y los días de internación, fueron similares a los reportados en la bibliografía, Sin embargo, las reinternaciones se presentaron en un número menor de pacientes y se asociaron mayoritariamente a FEY reducida.

Introduction: heart failure (HF) is a high prevalence clinical condition with a high morbidity and mortality rate. Knowledge of our records is an essential tool to approach this disease. Objetive: to analyze the record of patients admitted to the coronary care unit (CCU) at the Hospital Privado del Sur (HPS) in Bahía Blanca, who were diagnosed with HF. Materials and Methods: cross-sectional descriptive study of patients admitted at the CCU in the HPS from July 2016 to July 2017 with a discharge diagnosis of HF. From the CCU database the following variables were obtained: age, gender, cardiovascular risk factors (CRF), trigger causes, co-morbidities, ejection fraction (EF), need for inotropics and mechanical ventilation, number of days in hospital and number re-admissions. Statistical Analysis: In the qualitative variable analysis absolute and relative frequencies were analyzed. Median was used for quantitative variables. Statistical data collection and analysis was performed using the SPSS software. Results: 85 patients were registered, 58 males (68.2%); 70 (82.4%) older than 65 years; pathologies: 66 (77.6 %) presented HBP, 27 (31.8 %) diabetes, 37 (4.5 %) dyslipidemia, 30 (35.3 %) a history of Acute Myocardial Infarction (AMI), and 17 (20.0 %) auricular fibrillation. The most frequent triggers were dietary transgression, arrhythmia and infection. They presented Ejection Fractions (EF) lower than 40 %: 45 patients (52.9 %). The median of days in hospital was 5 (2-53). Seven (8.2%) patients were re-admitted into hospital, whose median was 1 (Score 1-5). Conclusions: Among triggers, cardiovascular risk factors and number of days in hospital were similar to those reported in the bibliography. However, re-admission rate was lower and mainly associated to reduced EF.

Uso de drogas de diseño en fiestas electrónicas: aspectos clínicos, neuropsiquiátricos, epidemiológicos y de intervención prehospitalaria / Use of designer drugs in electronic parties: clinical, neuropsychiatric, epidemiological and prehospital intervention aspects

Introducción: Las llamadas "fiestas electrónicas" permitieron la generalización del uso de drogas de diseño (DdD) para disminuir el cansancio, las inhibiciones y aumentar la empatía entre los asistentes. Objetivo: Presentar los resultados de prevalencia, aspectos neuropsiquiátricos, clínicos y epidemiológicos del uso de DdD en fiestas electrónicas. Material y método: Realizamos un estudio de campo, observacional y transversal, mediante encuesta estandarizada, normatizada y anónima para consignar parámetros clínicos y neuropsiquiátricos en 138 asistentes a fiestas electrónicas que mediante demanda espontánea o requerida por terceros fueron evaluados por médicos y refirieron consumo de DdD. Se aplicaron parámetros estadísticos y se cumplieron normas éticas. Resultados: Objetivamos un alto consumo de alcohol, con alta prevalencia de uso comórbido con otras sustancias de diseño como el éxtasis y GHB. Los consumidores de GHB presentaron disminución de parámetros clínicos con un síndrome inhibitorio, en contraste con los valores aumentados en usuarios de éxtasis. Conclusión: La diferencia en las manifestaciones clínico-neuropsiquiátricas tienen sustento en aspectos neurobiológicos del mecanismo de acción y condicionan el tratamiento prehospitalario.

Introduction: The so-called "electronic parties" allowed the widespread use of designer drugs (DOD) to reduce fatigue, inhibitions and increase empathy among participants. Objective: To present the results of prevalence, neuropsychiatric, clinical and epidemiological aspects of the use of DoD in electronic parties. Material and Methods: We conducted a field study, observational and transversal, through standardized, Normatized and anonymous survey to record clinical and neuropsychiatric parameters 138 attendees electronic parties that by spontaneous or required by third application were evaluated by physicians and reported consumption of DOD. Statistical parameters were applied and ethical standards were met. Results: We objectify a high consumption of alcohol, with high prevalence of comorbid use design with other substances like ecstasy and GHB. GHB consumers had decreased clinical parameters with an inhibitory syndrome, in contrast to the values increased in users of ecstasy. Conclusions The difference in clinical and neuropsychiatric manifestations have support in neurobiological aspects of the mechanism of action and determine the prehospital treatment.

Complejo QRS fragmentado: Utilidad como predictor de riesgo cardiovascular y correlación con trastornos regionales de motilidad y anatomía coronaria / Fragmented QRS complex: Usefulness as a predictor of cardiovascular risk and correlation with motility disorders and coronary anatomy / Complexo QRS fragmentado: Utilidade como preditor de risco cardiovascular e correlação com distúrbios de motilidade e anatomia coronariana

Introducción. El QRS fragmentado (QRSf) representa un retraso en la conducción ventricular causada por una cicatriz miocárdica y podría asociarse a un mayor riesgo de mortalidad y eventos arrítmicos. Objetivo. Correlacionar el QRSf con trastornos de motilidad regional y su asociación con el resultado de la cinecoronariografía y obtener el valor clínico del QRSf para identificar población de riesgo en el curso de un síndrome coronario agudo (SCA). Materiales y métodos. Se evaluaron retrospectivamente pacientes ingresados en nuestra institución con diagnóstico de SCA. Se buscó en el electrocardiograma (ECG) la presencia de QRSf y se lo relacionó con el compromiso de la motilidad parietal por ecocardiograma y con la anatomía coronaria. Resultados. Se analizaron 116 pacientes, edad promedio de 64,6 años (75%, hombres), el 78,2% correspondió a SCA sin elevación del segmento ST, de los cuales el 36% tuvo QRSf, al igual que el 68% de los que tuvieron elevación del ST. El 47,4% del total de pacientes presentó QRSf. En el 72% de los pacientes con QRSf se observó algún trastorno de motilidad parietal en el ecocardiograma (p< 0,03). En el 28% de los casos, se evidenció una correlación entre la región con QRSf en el ECG y la región hipoquinética o aquinética por ecocardiograma. La mortalidad global intrahospitalaria fue del 6,9%; de los cuales el 12,5% mostró QRSf. De los pacientes con enfermedad de más de un vaso, el 74,2% evidenció QRSf (p< 0,05), mientras que el 41% presentó enfermedad de tres vasos. Conclusiones. El QRSf se asoció de manera significativa a la presencia de enfermedad de más de un vaso y shock cardiogénico, pero no con la mortalidad. Encontramos una significativa asociación entre la presencia de trastornos regionales de contractilidad y la anatomía coronaria, pudiendo relacionar entre sí datos ecocardiográficos, hemodinámicos e imagenológicos.

Introduction. The fragmented QRS (fQRS) represents a ventricular conduction delay caused by a myocardial scar and may be associated with an increased risk of mortality and arrhythmics events. Objective. The fQRS correlate with regional motility disorders and their association with the outcome of coronary angiography and get fQRS clinical value to identify the population at risk in the course of an acute coronary syndrome (ACS). Materials and methods. It retrospectively evaluated patients admitted our institution with a diagnosis of ACS. Searched the electrocardiogram (ECG) the presence of fQRS and related it to the commitment of wall motion by echocardiography and coronary anatomy. Results. There were evaluated 116 patients, average age 64.6% years (75% male). The 78.2% corresponded to ACS without ST segment elevation, of which 36% had fQRS, like 68% that had ST elevation. Among the whole of patients, the 47.4% presented fQRS. In 72% of patients showed some kind of parietal motility disorders in echocardiogram (p< 0.03). The 28% of remaining cases had demonstrated the correlation between the wall with fQRS in the ECG and the hypokinetic or akinetic area by echocardiogram. The in-hospital global mortality was of 6.9%, of which 12.5% had fQRS. Among the patients with more than one vessel disease, 74.2% showed fQRS (p< 0.05) and 41% presented three vessels disease. Conclusions. The fQRS was associated significantly with the presence of more than one vessel disease and cardiogenic shock, but not with mortality. We found a huge association between the presence of regional contractility disorders and coronary anatomy, making possible the relationship between echocardiographic, hemodynamic and imaging data.

Introdução. O QRS fragmentado (QRSf) representa um atraso de condução ventricular causada por uma cicatriz do miocárdio e pode estar associada com um risco aumentado de mortalidade e de eventos arrítmicos. Objetivo. Correlacionar o QRSf com distúrbios de motilidade regionais e sua associação com o resultado da angiografia coronária e obter o valor clínico do QRSf para identificar populações em risco no curso de uma síndrome coronária aguda (SCA). Materiais e métodos. Foram avaliados retrospectivamente os pacientes internados em nossa instituição com um diagnóstico de SCA. Procurando a presença de QRSf no ECG e relacionado para o compromisso do movimento da parede por meio de ecocardiografia e anatomia coronária. Resultados. Foram avaliados 116 pacientes, com idade média de 64,6 anos (75% do sexo masculino), O 78,2% corresponderam a SCA sem elevação do segmento ST, dos quais 36% tinham QRSf, como 68% dos que tinham elevação de ST. O 47,4% dos pacientes presenteou QRSf. Em 72% dos pacientes com QRSf apresentavam algum transtorno de movimento da parede no ecocardiograma (p< 0,03). Em 28% dos casos foi encontrada uma correlação entre a região com QRSf no ECG e a região hipoquinética ou região acinético pelo ecocardiograma. Mortalidade hospitalar global foi do 6,9%, dos quais 12,5% com QRSf. Dos pacientes com doença de mais de um vaso arterial, o 74,2% apresentaram QRSf (p< 0,05), enquanto que o 41% tinham doença de três vasos. Conclusões. O QRSf significativamente associados com a presença da doença de mais de um vaso arterial e choque cardiogénico, mas não com a mortalidade. Encontramos uma associação significativa entre a presença de distúrbios de contratilidade regional e anatomia coronária, tornando possível a relação entre dados ecocardiográficos, hemodinâmicos e os de imagem.

Complejo QRS fragmentado: Utilidad como predictor de riesgo cardiovascular y correlación con trastornos regionales de motilidad y anatomía coronaria / Fragmented QRS complex: Usefulness as a predictor of cardiovascular risk and correlation with motility disorders and coronary anatomy / Complexo QRS fragmentado: Utilidade como preditor de risco cardiovascular e correlaþÒo com distúrbios de motilidade e anatomia coronariana

Introducción. El QRS fragmentado (QRSf) representa un retraso en la conducción ventricular causada por una cicatriz miocárdica y podría asociarse a un mayor riesgo de mortalidad y eventos arrítmicos. Objetivo. Correlacionar el QRSf con trastornos de motilidad regional y su asociación con el resultado de la cinecoronariografía y obtener el valor clínico del QRSf para identificar población de riesgo en el curso de un síndrome coronario agudo (SCA). Materiales y métodos. Se evaluaron retrospectivamente pacientes ingresados en nuestra institución con diagnóstico de SCA. Se buscó en el electrocardiograma (ECG) la presencia de QRSf y se lo relacionó con el compromiso de la motilidad parietal por ecocardiograma y con la anatomía coronaria. Resultados. Se analizaron 116 pacientes, edad promedio de 64,6 años (75%, hombres), el 78,2% correspondió a SCA sin elevación del segmento ST, de los cuales el 36% tuvo QRSf, al igual que el 68% de los que tuvieron elevación del ST. El 47,4% del total de pacientes presentó QRSf. En el 72% de los pacientes con QRSf se observó algún trastorno de motilidad parietal en el ecocardiograma (p< 0,03). En el 28% de los casos, se evidenció una correlación entre la región con QRSf en el ECG y la región hipoquinética o aquinética por ecocardiograma. La mortalidad global intrahospitalaria fue del 6,9%; de los cuales el 12,5% mostró QRSf. De los pacientes con enfermedad de más de un vaso, el 74,2% evidenció QRSf (p< 0,05), mientras que el 41% presentó enfermedad de tres vasos. Conclusiones. El QRSf se asoció de manera significativa a la presencia de enfermedad de más de un vaso y shock cardiogénico, pero no con la mortalidad. Encontramos una significativa asociación entre la presencia de trastornos regionales de contractilidad y la anatomía coronaria, pudiendo relacionar entre sí datos ecocardiográficos, hemodinámicos e imagenológicos.(AU)

Introduction. The fragmented QRS (fQRS) represents a ventricular conduction delay caused by a myocardial scar and may be associated with an increased risk of mortality and arrhythmics events. Objective. The fQRS correlate with regional motility disorders and their association with the outcome of coronary angiography and get fQRS clinical value to identify the population at risk in the course of an acute coronary syndrome (ACS). Materials and methods. It retrospectively evaluated patients admitted our institution with a diagnosis of ACS. Searched the electrocardiogram (ECG) the presence of fQRS and related it to the commitment of wall motion by echocardiography and coronary anatomy. Results. There were evaluated 116 patients, average age 64.6% years (75% male). The 78.2% corresponded to ACS without ST segment elevation, of which 36% had fQRS, like 68% that had ST elevation. Among the whole of patients, the 47.4% presented fQRS. In 72% of patients showed some kind of parietal motility disorders in echocardiogram (p< 0.03). The 28% of remaining cases had demonstrated the correlation between the wall with fQRS in the ECG and the hypokinetic or akinetic area by echocardiogram. The in-hospital global mortality was of 6.9%, of which 12.5% had fQRS. Among the patients with more than one vessel disease, 74.2% showed fQRS (p< 0.05) and 41% presented three vessels disease. Conclusions. The fQRS was associated significantly with the presence of more than one vessel disease and cardiogenic shock, but not with mortality. We found a huge association between the presence of regional contractility disorders and coronary anatomy, making possible the relationship between echocardiographic, hemodynamic and imaging data.(AU)

IntroduþÒo. O QRS fragmentado (QRSf) representa um atraso de conduþÒo ventricular causada por uma cicatriz do miocárdio e pode estar associada com um risco aumentado de mortalidade e de eventos arrítmicos. Objetivo. Correlacionar o QRSf com distúrbios de motilidade regionais e sua associaþÒo com o resultado da angiografia coronária e obter o valor clínico do QRSf para identificar populaþ§es em risco no curso de uma síndrome coronária aguda (SCA). Materiais e métodos. Foram avaliados retrospectivamente os pacientes internados em nossa instituiþÒo com um diagnóstico de SCA. Procurando a presenþa de QRSf no ECG e relacionado para o compromisso do movimento da parede por meio de ecocardiografia e anatomia coronária. Resultados. Foram avaliados 116 pacientes, com idade média de 64,6 anos (75% do sexo masculino), O 78,2% corresponderam a SCA sem elevaþÒo do segmento ST, dos quais 36% tinham QRSf, como 68% dos que tinham elevaþÒo de ST. O 47,4% dos pacientes presenteou QRSf. Em 72% dos pacientes com QRSf apresentavam algum transtorno de movimento da parede no ecocardiograma (p< 0,03). Em 28% dos casos foi encontrada uma correlaþÒo entre a regiÒo com QRSf no ECG e a regiÒo hipoquinética ou regiÒo acinético pelo ecocardiograma. Mortalidade hospitalar global foi do 6,9%, dos quais 12,5% com QRSf. Dos pacientes com doenþa de mais de um vaso arterial, o 74,2% apresentaram QRSf (p< 0,05), enquanto que o 41% tinham doenþa de trìs vasos. Conclus§es. O QRSf significativamente associados com a presenþa da doenþa de mais de um vaso arterial e choque cardiogénico, mas nÒo com a mortalidade. Encontramos uma associaþÒo significativa entre a presenþa de distúrbios de contratilidade regional e anatomia coronária, tornando possível a relaþÒo entre dados ecocardiográficos, hemodinÔmicos e os de imagem.(AU)

Estreptoquinasa en infusión lenta versus infusión rápida en tromboembolismo pulmonar agudo de alto riesgo. Comparación de 3 casos clínicos. / Slow vs. rapid infusion streptokinase in high risk acute pulmonary thromboembolism. A comparison of 3 clinical cases

Presentamos tres casos clínicos ingresados en el servicio de Unidad Coronaria (UCO) del Hospital Privado de Sur de Bahía Blanca con diagnóstico de Tromboembolismo pulmonar (TEP) de alto riesgo tratados con Estreptoquinasa (EQ), dos de los pacientes recibieron infusión lenta y uno infusión rápida. El objetivo es evaluar comparativamente parámetros clínicos, hemodinámicos y complicaciones entre ambas formas infusión.

Three patients are admitted at the Coronary Care Unit (CCU) of the Hospital Privado del Sur of the city of Bahía Blanca, with a diagnosis of high risk Pulmonary Thromboembolism (PTE). Two of them were treated with slow infusion streptokinase and the other one with rapid infusion streptokinase. The objective was to assess and compare clinical and hemodynamic parameters as well as complications between both infusion types.

MOWServ: a web client for integration of bioinformatic resources.

The productivity of any scientist is affected by cumbersome, tedious and time-consuming tasks that try to make the heterogeneous web services compatible so that they can be useful in their research. MOWServ, the bioinformatic platform offered by the Spanish National Institute of Bioinformatics, was released to provide integrated access to databases and analytical tools. Since its release, the number of available services has grown dramatically, and it has become one of the main contributors of registered services in the EMBRACE Biocatalogue. The ontology that enables most of the web-service compatibility has been curated, improved and extended. The service discovery has been greatly enhanced by Magallanes software and biodataSF. User data are securely stored on the main server by an authentication protocol that enables the monitoring of current or already-finished user's tasks, as well as the pipelining of successive data processing services. The BioMoby standard has been greatly extended with the new features included in the MOWServ, such as management of additional information (metadata such as extended descriptions, keywords and datafile examples), a qualified registry, error handling, asynchronous services and service replication. All of them have increased the MOWServ service quality, usability and robustness. MOWServ is available at http://www.inab.org/MOWServ/ and has a mirror at http://www.bitlab-es.com/MOWServ/.

jORCA: easily integrating bioinformatics Web Services.

MOTIVATION: Web services technology is becoming the option of choice to deploy bioinformatics tools that are universally available. One of the major strengths of this approach is that it supports machine-to-machine interoperability over a network. However, a weakness of this approach is that various Web Services differ in their definition and invocation protocols, as well as their communication and data formats-and this presents a barrier to service interoperability. RESULTS: jORCA is a desktop client aimed at facilitating seamless integration of Web Services. It does so by making a uniform representation of the different web resources, supporting scalable service discovery, and automatic composition of workflows. Usability is at the top of the jORCA agenda; thus it is a highly customizable and extensible application that accommodates a broad range of user skills featuring double-click invocation of services in conjunction with advanced execution-control, on the fly data standardization, extensibility of viewer plug-ins, drag-and-drop editing capabilities, plus a file-based browsing style and organization of favourite tools. The integration of bioinformatics Web Services is made easier to support a wider range of users. .